WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EAAT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87MG.IHC
Rat brain was stained with Anti-EAAT1 rabbit antibodyIHC
Mouse brain was stained with Anti-EAAT1 rabbit antibody.IHC
Human brain was stained with Anti-EAAT1 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A:EAAT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | EAAT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-2000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC1A3 |
|---|---|
| Gene Synonyms | EA6 EAAT1 GLAST GLAST1 |
| Gene Full Name | solute carrier family 1 member 3 |
| Gene Summary | This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014] |
| Alternative Names | SLC1A3 EAAT1 GLAST GLAST1 Excitatory amino acid transporter 1 Sodium-dependent glutamate/aspartate transporter 1 GLAST-1 Solute carrier family 1 member 3 |
| Molecular Weight(MW) | 60kDa(Observed MW 59kDa) |
| Cellular Localization | Cell membrane |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EAAT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87MG.
IHC
Rat brain was stained with Anti-EAAT1 rabbit antibody
IHC
Mouse brain was stained with Anti-EAAT1 rabbit antibody.
IHC
Human brain was stained with Anti-EAAT1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A:EAAT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:500-2000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
