WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:10000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: PC-12 - Rat adrenal pheochromocytoma Lane2: 3T3-L1 - Mouse embryonic fibroblast Lane3: PC-3 - Human prostate adenocarcinoma Lane4: U-14 - Mouse cervical carcinoma Lane5: 293F - HEK293 derivative, adapted for suspension culture.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: 3T3-L1 Lane 3: K562 Lane 4: C6 .WB
Western blot analysis of lysates from Hela WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human kidney was stained with anti-Histone H3 rabbit antibody.IHC
Mouse colon was stained with anti-Histone H3 rabbit antibody.IHC
Rat colon was stained with anti-Histone H3 rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H3 Antibody(red) was diluted at 1:200(4° overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).ICC/IF
Rat liver was stained with anti-Histone H3 Rabbit antibody.ICC/IF
Immunofluorescence analysis of HeLa. Picture A: Histone H3 Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Histone H3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:20000 ICC/IF:1:200-1:1000 IHC:1:5000-1:20000 |
|
| Species Reactivity | CatHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H3C1 |
|---|---|
| Gene Synonyms | H3/A H3FA HIST1H3A |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3 |
| Molecular Weight(MW) | 15kDa(Observed MW 15kDa) |
| Cellular Localization | Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:10000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: PC-12 - Rat adrenal pheochromocytoma Lane2: 3T3-L1 - Mouse embryonic fibroblast Lane3: PC-3 - Human prostate adenocarcinoma Lane4: U-14 - Mouse cervical carcinoma Lane5: 293F - HEK293 derivative, adapted for suspension culture.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: 3T3-L1 Lane 3: K562 Lane 4: C6 .
WB
Western blot analysis of lysates from Hela WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-Histone H3 rabbit antibody.
IHC
Mouse colon was stained with anti-Histone H3 rabbit antibody.
IHC
Rat colon was stained with anti-Histone H3 rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H3 Antibody(red) was diluted at 1:200(4° overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
ICC/IF
Rat liver was stained with anti-Histone H3 Rabbit antibody.
ICC/IF
Immunofluorescence analysis of HeLa. Picture A: Histone H3 Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:5000-1:20000 ICC/IF:1:200-1:1000 IHC:1:5000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
