WB
Western Blot analysis using HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α rabbit mAb diluted at 1:2000. anti-AKT (Phospho Ser473) Rabbit mAb diluted at 1:2000. Loading contrl: Mouse anti GAPDH(1:5000) Secondary : Dylight 800, Goat Anti Rabbit IgG(1:10000) Dylight 680, Goat Anti Rabbit IgG (1:10000).WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse brain Lane 4: Rat brain.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.WB
Western blot analysis of lysates from HL60 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with PI3-Kinase p85 α rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Mouse testis was stained with Anti-PI3-Kinase p85 α rabbit Antibody.IHC
Human placenta was stained with Anti-PI3-Kinase p85 α rabbit Antibody.ICC/IF
Immunofluorescence analysis of Human placenta.ICC/IF
Immunofluorescence analysis of MCF7 cell. 1,primary Antibody was diluted at 1:100(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).| Product Name | PI 3 Kinase P85α Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-5000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|
| Species Reactivity | GoatHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | p85 AGM7 GRB1 IMD36 p85alpha p85-ALPHA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Alternative Names | PIK3R1 GRB1 Phosphatidylinositol 3-kinase regulatory subunit alpha PI3-kinase regulatory subunit alpha PI3K regulatory subunit alpha PtdIns-3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PI3-kinase subunit p85-alpha PtdIns-3-kinase regulatory subunit p85-alpha |
| Molecular Weight(MW) | 84kDa(Observed MW 84kDa) |
| Cellular Localization | nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane |
WB
Western Blot analysis using HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α rabbit mAb diluted at 1:2000. anti-AKT (Phospho Ser473) Rabbit mAb diluted at 1:2000. Loading contrl: Mouse anti GAPDH(1:5000) Secondary : Dylight 800, Goat Anti Rabbit IgG(1:10000) Dylight 680, Goat Anti Rabbit IgG (1:10000).
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse brain Lane 4: Rat brain.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.
WB
Western blot analysis of lysates from HL60 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with PI3-Kinase p85 α rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Mouse testis was stained with Anti-PI3-Kinase p85 α rabbit Antibody.
IHC
Human placenta was stained with Anti-PI3-Kinase p85 α rabbit Antibody.
ICC/IF
Immunofluorescence analysis of Human placenta.
ICC/IF
Immunofluorescence analysis of MCF7 cell. 1,primary Antibody was diluted at 1:100(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).| Application Notes | WB:1:1000-5000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
