WB
Western blot analysis of anti- ITGB2 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- ITGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody. CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-CD18/ITGB2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis using anti- ITGB2 antibody. detected in paraffin-embedded section of human tonsil tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)and counterstained with DAPI (blue).| Product Name | Anti-CD18/ITGB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGB2 |
|---|---|
| Gene Synonyms | LAD CD18 MF17 MFI7 LCAMB LFA-1 MAC-1 |
| Gene Full Name | integrin subunit beta 2 |
| Gene Summary | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | CD18 Integrin beta 2 ITGB2 LAD LCAMB LFA 1 MAC 1 MF17 MFI7 |
| Molecular Weight(MW) | 85kDa(Observed MW 90-100kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of anti- ITGB2 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- ITGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody. CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-CD18/ITGB2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis using anti- ITGB2 antibody. detected in paraffin-embedded section of human tonsil tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)and counterstained with DAPI (blue).| Application Notes | WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
