WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRDX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRDX5 (1:2000).WB
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PRDX5 monoclonal antibody (1:500).IHC
Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-PRDX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min) (1:250)| Product Name | Anti-PRX5/PRDX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human PRDX5 produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:250 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDX5 |
|---|---|
| Gene Synonyms | PLP ACR1 B166 PRXV PMP20 PRDX6 prx-V SBBI10 AOEB166 HEL-S-55 |
| Gene Full Name | peroxiredoxin 5 |
| Gene Summary | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene. [provided by RefSeq, Nov 2017] |
| Alternative Names | ACR1 Alu corepressor 1 Antioxidant enzyme B166 AOEB166 AOPP B166 Liver tissue 2D page spot 71B Peroxiredoxin 5 Peroxiredoxin 5 mitochondrial Peroxiredoxin V Peroxisomal antioxidant enzyme PLP PMP20 PRDX5 Prx V PRX5 PRXV SBBI10 Thioredoxin peroxidase PMP20 Thioredoxin reductase TPx type VI |
| Molecular Weight(MW) | 22kDa |
| Cellular Localization | Mitochondrion |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRDX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRDX5 (1:2000).
WB
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PRDX5 monoclonal antibody (1:500).
IHC
Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-PRDX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min) (1:250)| Application Notes | WB:1:500-2000 IHC:1:250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for 12 months from date of receipt. Store at -20°C as received. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
