WB
Western blot analysis of anti- SA1/STAG1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- SA1/STAG1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.ICC/IF
ICC/IF analysis of STAG1 using anti-STAG1 antibody and anti-Alpha Tubulin antibody. STAG1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-STAG1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Flow Cytometry analysis of U937 cells using anti-STAG1 antibody. Overlay histogram showing U937 cells stained with anti-STAG1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-STAG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SA1/STAG1 recombinant protein (Position: Q748-L1224). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAG1 |
|---|---|
| Gene Synonyms | SA1 MRD47 SCC3A |
| Gene Full Name | STAG1 cohesin complex component |
| Gene Summary | This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008] |
| Alternative Names | Cohesin subunit SA 1 SA1 SCC3 homolog 1 STAG1 stromal antigen 1 |
| Molecular Weight(MW) | 144kDa(Observed MW 155kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of anti- SA1/STAG1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- SA1/STAG1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
ICC/IF
ICC/IF analysis of STAG1 using anti-STAG1 antibody and anti-Alpha Tubulin antibody. STAG1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-STAG1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Flow Cytometry analysis of U937 cells using anti-STAG1 antibody. Overlay histogram showing U937 cells stained with anti-STAG1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
