WB
Western blot analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-L-Plastin/LCP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. L-Plastin/LCP1 was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-L-Plastin/LCP1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of U2OS cells using anti-LCP1 antibody.Overlay histogram showing U2OS cells stained with anti-LCP1 antibody (Blue line).Fluoro®488 conjugated goat anti-rabbit IgG was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.ICC/IF
ICC/IF analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. L-Plastin/LCP1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-L-Plastin/LCP1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Anti-L-Plastin/LCP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human Plastin L/LCP1 recombinant protein (M1-K444). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LCP1 |
|---|---|
| Gene Synonyms | CP64 PLS2 LC64P HEL-S-37 L-PLASTIN |
| Gene Full Name | lymphocyte cytosolic protein 1 |
| Gene Summary | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008] |
| Alternative Names | L PLASTIN LCP 1 LCP1 L-Plastin Lymphocyte cytosolic protein 1 Plastin 2 PLS2 |
| Molecular Weight(MW) | 70kDa(Observed MW 70kDa) |
| Cellular Localization | Cytoplasm |
WB
Western blot analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-L-Plastin/LCP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. L-Plastin/LCP1 was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-L-Plastin/LCP1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of U2OS cells using anti-LCP1 antibody.Overlay histogram showing U2OS cells stained with anti-LCP1 antibody (Blue line).Fluoro®488 conjugated goat anti-rabbit IgG was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ICC/IF
ICC/IF analysis of L-Plastin/LCP1 using anti-L-Plastin/LCP1 antibody. L-Plastin/LCP1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-L-Plastin/LCP1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
