WB
Western blot analysis of NEDD4 using anti-NEDD4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: U251 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: Hacat whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U87 cells using anti-NEDD4 antibody. Overlay histogram showing U87 cells stained with anti-NEDD4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-NEDD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NEDD4 recombinant protein (Position: M1-A736). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEDD4 |
|---|---|
| Gene Synonyms | RPF1 NEDD4-1 |
| Gene Full Name | NEDD4 E3 ubiquitin protein ligase |
| Gene Summary | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016] |
| Alternative Names | KIAA0093 NEDD 4 NEDD4 NEDD4 1 RPF1 |
| Molecular Weight(MW) | 149kDa(Observed MW 105-150kDa) |
| Cellular Localization | membrane |
WB
Western blot analysis of NEDD4 using anti-NEDD4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: U251 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: Hacat whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of NEDD4 using anti-NEDD4 antibody. NEDD4 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NEDD4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U87 cells using anti-NEDD4 antibody. Overlay histogram showing U87 cells stained with anti-NEDD4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
