WB
Western blot analysis of ZIP8/SLC39A8 using anti-ZIP8/SLC39A8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ZIP8/SLC39A8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ZIP8/SLC39A8 using anti-ZIP8/SLC39A8 antibody. ZIP8/SLC39A8 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with rabbit anti-ZIP8/SLC39A8 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of HEL cells using anti-ZIP8/SLC39A8 antibody. Overlay histogram showing HEL cells stained with anti-ZIP8/SLC39A8 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIP8/SLC39A8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-ZIP8/SLC39A8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ZIP8/SLC39A8 recombinant protein (Position: E23-A438). Human ZIP8/SLC39A8 shares 89.5% and 87.8% amino acid (aa) sequence identity with mouse and rat ZIP8/SLC39A8, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-2000 IHC:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A8 |
|---|---|
| Gene Synonyms | ZIP8 CDG2N PP3105 BIGM103 LZT-Hs6 |
| Gene Full Name | solute carrier family 39 member 8 |
| Gene Summary | This gene encodes a member of the SLC39 family of solute-carrier genes, which show structural characteristics of zinc transporters. The encoded protein is glycosylated and found in the plasma membrane and mitochondria, and functions in the cellular import of zinc at the onset of inflammation. It is also thought to be the primary transporter of the toxic cation cadmium, which is found in cigarette smoke. Multiple transcript variants encoding different isoforms have been found for this gene. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Oct 2008] |
| Alternative Names | BIGM103 PP3105 SLC39A8 ZIP 8 ZIP8 |
| Molecular Weight(MW) | 50kDa(Observed MW 80kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of ZIP8/SLC39A8 using anti-ZIP8/SLC39A8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ZIP8/SLC39A8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ZIP8/SLC39A8 using anti-ZIP8/SLC39A8 antibody. ZIP8/SLC39A8 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with rabbit anti-ZIP8/SLC39A8 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of HEL cells using anti-ZIP8/SLC39A8 antibody. Overlay histogram showing HEL cells stained with anti-ZIP8/SLC39A8 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIP8/SLC39A8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
