WB
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: SiHa cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (30 µg/Lane), Lane 2: NIH/3T3 cell lysate (30 µg/Lane), Lane 3: RAW264.7 cell lysate (20 µg/Lane), Lane 4: PC-12 cell lysate (26 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | SLC39A8 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Dnmt1 aa 500-600 / 1616. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC39A8 |
|---|---|
| Gene Synonyms | ZIP8 CDG2N PP3105 BIGM103 LZT-Hs6 |
| Gene Full Name | solute carrier family 39 member 8 |
| Gene Summary | This gene encodes a member of the SLC39 family of solute-carrier genes, which show structural characteristics of zinc transporters. The encoded protein is glycosylated and found in the plasma membrane and mitochondria, and functions in the cellular import of zinc at the onset of inflammation. It is also thought to be the primary transporter of the toxic cation cadmium, which is found in cigarette smoke. Multiple transcript variants encoding different isoforms have been found for this gene. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Oct 2008] |
| Alternative Names | 4933419D20Rik antibody AA986696 antibody BCG induced integral membrane protein 103 antibody BCG induced integral membrane protein BIGMo-103 (Up-regulated by BCG-CWS) (Hypothetical protein) antibody LIV-1 subfamily of ZIP zinc transporter 6 antibody LZT Hs6 antibody MGC108852 antibody PP3105 antibody SLC39A8 antibody Solute carrier family 39 (zinc transporter) member 8 antibody 4933419D20Rik antibody AA986696 antibody BCG induced integral membrane protein 103 antibody BCG induced integral membrane protein BIGMo-103 (Up-regulated by BCG-CWS) (Hypothetical protein) antibody LIV-1 subfamily of ZIP zinc transporter 6 antibody LZT Hs6 antibody MGC108852 antibody PP3105 antibody SLC39A8 antibody Solute carrier family 39 (zinc transporter) member 8 antibody Solute carrier family 39 (zinc transporter) member 8 (SLC39A8) antibody Solute carrier family 39 member 8 antibody zinc transporter ZIP8 antibody ZIP8 antibody Zrt- and Irt-like protein 8 antibody |
| Molecular Weight(MW) | 50kDa(Observed band size: 65/130 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: SiHa cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (30 µg/Lane), Lane 2: NIH/3T3 cell lysate (30 µg/Lane), Lane 3: RAW264.7 cell lysate (20 µg/Lane), Lane 4: PC-12 cell lysate (26 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
