WB
Western blot analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: COS-7 whole cell lysates, Lane 3: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC25A51/52 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. SLC25A51/52 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with rabbit anti-SLC25A51/52 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. SLC25A51/52 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SLC25A51/52 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U251 cells using anti-SLC25A51/52 antibody. Overlay histogram showing U251 cells stained with anti-SLC25A51/52 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC25A51/52 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-SLC25A51/52 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SLC25A51/52 recombinant protein (Position: M1-E291). Human SLC25A51/52 shares 87% and 86.3% amino acid (aa) sequence identity with mouse and rat SLC25A51/52, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMonkeyMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC25A51 |
|---|---|
| Gene Synonyms | CG7943 MCART1 |
| Gene Full Name | solute carrier family 25 member 51 |
| Gene Summary | Enables NAD transmembrane transporter activity. Involved in mitochondrial NAD transmembrane transport. Located in mitochondrion. Is active in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MCART1 SLC25A51 MCART2 SLC25A52 |
| Molecular Weight(MW) | 34kDa(Observed MW 45kDa) |
| Cellular Localization | Mitochondrion inner membrane |
WB
Western blot analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: COS-7 whole cell lysates, Lane 3: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC25A51/52 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. SLC25A51/52 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. The tissue section was incubated with rabbit anti-SLC25A51/52 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SLC25A51/52 using anti-SLC25A51/52 antibody. SLC25A51/52 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SLC25A51/52 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U251 cells using anti-SLC25A51/52 antibody. Overlay histogram showing U251 cells stained with anti-SLC25A51/52 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC25A51/52 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
