WB
Western blot analysis of SEL1L using anti-SEL1L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).IF-P
IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).ICC/IF
ICC/IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HEL cells using anti-SEL1L antibody. Overlay histogram showing HEL cells stained with anti-SEL1L antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEL1L Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-SEL1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SEL1L recombinant protein (Position: E105-E723). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEL1L |
|---|---|
| Gene Synonyms | Hrd3 SEL1L1 NEDGSAF NEDHGFA PRO1063 SEL1-LIKE |
| Gene Full Name | SEL1L adaptor subunit of SYVN1 ubiquitin ligase |
| Gene Summary | The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | IBD2 PRO1063 Protein sel 1 homolog 1 Sel 1L SEL1 LIKE SEL1L TSA305 |
| Molecular Weight(MW) | 89kDa(Observed MW 100kDa) |
| Cellular Localization | Endoplasmic reticulum membrane |
WB
Western blot analysis of SEL1L using anti-SEL1L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
IF-P
IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
ICC/IF
ICC/IF analysis of SEL1L using anti-SEL1L antibody. SEL1L was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SEL1L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HEL cells using anti-SEL1L antibody. Overlay histogram showing HEL cells stained with anti-SEL1L antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEL1L Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
