WB
Western Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DOHH rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.| Product Name | DOHH Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DOHH |
|---|---|
| Gene Synonyms | HLRC1 hDOHH NEDMVIC |
| Gene Full Name | deoxyhypusine hydroxylase |
| Gene Summary | This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009] |
| Alternative Names | DOHH HLRC1 Deoxyhypusine hydroxylase hDOHH Deoxyhypusine dioxygenase Deoxyhypusine monooxygenase HEAT-like repeat-containing protein 1 |
| Molecular Weight(MW) | Observed MW 33kDa |
WB
Western Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DOHH rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.| Application Notes | WB:1:1000-5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
