WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RPA32/RPA2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Hela Lane 3: C6 Lane 4: U-14.IHC
Human kidney was stained with anti-RPA32/RPA2 rabbit antibody.IHC
Mouse liver was stained with anti-RPA32/RPA2 rabbit antibody.IHC
Rat liver was stained with anti-RPA32/RPA2 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: RPA32/RPA2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | RPA32/RPA2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Phospho |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPA2 |
|---|---|
| Gene Synonyms | REPA2 RPA32 RP-A p32 RP-A p34 |
| Gene Full Name | replication protein A2 |
| Gene Summary | This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017] |
| Alternative Names | RPA2 REPA2 RPA32 RPA34 Replication protein A 32 kDa subunit RP-A p32 Replication factor A protein 2 RF-A protein 2 Replication protein A 34 kDa subunit RP-A p34 |
| Molecular Weight(MW) | 29kDa(Observed MW 32kDa) |
| Cellular Localization | Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RPA32/RPA2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Hela Lane 3: C6 Lane 4: U-14.
IHC
Human kidney was stained with anti-RPA32/RPA2 rabbit antibody.
IHC
Mouse liver was stained with anti-RPA32/RPA2 rabbit antibody.
IHC
Rat liver was stained with anti-RPA32/RPA2 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: RPA32/RPA2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
