WB
Western blot analysis of NPAS4L using anti-NPAS4L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NPAS4L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Anti-Zebrafish NPAS4L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish NPAS4L recombinant protein (Position: Q94-I647) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | npas4l |
|---|---|
| Gene Synonyms | clo |
| Gene Full Name | neuronal PAS domain protein 4 like |
| Gene Summary | This gene encodes a PAS-domain-containing basic helix-loop-helix transcription factor. This gene is expressed during late gastrulation and functions as an essential transcriptional regulator of mesoderm diversification and differentiation. The encoded protein is involved in directing the specification of endothelial and haematopoietic cell lineages. Mutations in this genes result in a loss of endothelial and haematopoietic cells and an increase in cardiomyocyte numbers. This gene is located in the telomeric region of chromosome 13. [provided by RefSeq, Aug 2016] |
| Molecular Weight(MW) | 69kDa(Observed MW 90kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of NPAS4L using anti-NPAS4L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NPAS4L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
