WB
Western blot analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: 293T whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MSK2/RPS6KA4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of K562 cells using anti-MSK2/RPS6KA4 antibody. Overlay histogram showing K562 cells stained with anti-MSK2/RPS6KA4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSK2/RPS6KA4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-MSK2/RPS6KA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MSK2/RSK-B/RPS6KA4 recombinant protein (Position: Q302-S772). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA4 |
|---|---|
| Gene Synonyms | MSK2 RSK-B S6K-alpha-4 |
| Gene Full Name | ribosomal protein S6 kinase A4 |
| Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| Alternative Names | MSK2 RPS6KA4 RSKB S6K-alpha-4 |
| Molecular Weight(MW) | 86kDa(Observed MW 86kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: 293T whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MSK2/RPS6KA4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of MSK2/RPS6KA4 using anti-MSK2/RPS6KA4 antibody. MSK2/RPS6KA4 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-MSK2/RPS6KA4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of K562 cells using anti-MSK2/RPS6KA4 antibody. Overlay histogram showing K562 cells stained with anti-MSK2/RPS6KA4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSK2/RPS6KA4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
