WB
Western blot analysis of various lysates using Aconitase 2 (ACO2) Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.WB
Western blot analysis of lysates from Rat kidney, using Aconitase 2 (ACO2) Rabbit pAb at 1:2000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using Aconitase 2(ACO2) Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingAconitase 2 (ACO2) Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using Aconitase 2(ACO2) Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.ICC/IF
Immunofluorescence analysis of A-431 cellsusing Aconitase 2 (ACO2) Rabbit pAb at a dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of HeLa cellsusing Aconitase 2 (ACO2) Rabbit pAb at a dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Aconitase 2 (ACO2) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:200 ICC/IF:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACO2 |
|---|---|
| Gene Synonyms | ICRD OCA8 OPA9 ACONM HEL-S-284 |
| Gene Full Name | aconitase 2 |
| Gene Summary | The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008] |
| Alternative Names | ICRD OCA8 OPA9 ACONM HEL-S-284 Aconitase 2 (ACO2) |
| Molecular Weight(MW) | 85kDa(Observed band size:85kDa) |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of various lysates using Aconitase 2 (ACO2) Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.
WB
Western blot analysis of lysates from Rat kidney, using Aconitase 2 (ACO2) Rabbit pAb at 1:2000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using Aconitase 2(ACO2) Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingAconitase 2 (ACO2) Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using Aconitase 2(ACO2) Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
ICC/IF
Immunofluorescence analysis of A-431 cellsusing Aconitase 2 (ACO2) Rabbit pAb at a dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing Aconitase 2 (ACO2) Rabbit pAb at a dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:200 ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
