WB
Western blot analysis of lysates from wild type (WT) and Abhd6 knockout (KO) Mouse brain using [KOValidated] Abhd6 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.WB
Western blot analysis of various lysates using [KO Validated] Abhd6 Rabbit mAb at 1:6000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.WB
Western blot analysis of lysates from RPMI 8226 cells using [KO Validated] Abhd6 Rabbit mAb at1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using [KOValidated] Abhd6 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IF-F
Confocal imaging of frozen sections Mousebrain tissue using [KO Validated] Abhd6Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). Microwave antigen retrievalperformed with 0.01M Citrate Buffer (pH 6.0)prior to IF staining. Objective: 40x.| Product Name | [KO Validated] Abhd6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:6000-1:24000 IHC:1:100-1:400 IF-F:1:100-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Abhd6 |
|---|---|
| Gene Synonyms | 0610041D24Rik |
| Gene Full Name | abhydrolase domain containing 6 |
| Gene Summary | Enables monoacylglycerol lipase activity and phospholipase activity. Involved in several processes, including monoacylglycerol catabolic process; negative regulation of cold-induced thermogenesis; and phospholipid metabolic process. Acts upstream of or within long-term synaptic depression; negative regulation of cell migration; and regulation of endocannabinoid signaling pathway. Located in late endosome membrane; lysosomal membrane; and mitochondrion. Part of AMPA glutamate receptor complex. Is active in GABA-ergic synapse; glutamatergic synapse; and postsynaptic membrane. Is expressed in cerebral cortex ventricular layer and cortical plate. Orthologous to human ABHD6 (abhydrolase domain containing 6, acylglycerol lipase). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | AA673485 AV065425 0610041D24Rik ABHD6 |
| Molecular Weight(MW) | 38kDa(Observed band size:38kDa) |
| Cellular Localization | Late endosome membrane, Lysosome membrane, Mitochondrion membrane. |
WB
Western blot analysis of lysates from wild type (WT) and Abhd6 knockout (KO) Mouse brain using [KOValidated] Abhd6 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
WB
Western blot analysis of various lysates using [KO Validated] Abhd6 Rabbit mAb at 1:6000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
WB
Western blot analysis of lysates from RPMI 8226 cells using [KO Validated] Abhd6 Rabbit mAb at1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using [KOValidated] Abhd6 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IF-F
Confocal imaging of frozen sections Mousebrain tissue using [KO Validated] Abhd6Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). Microwave antigen retrievalperformed with 0.01M Citrate Buffer (pH 6.0)prior to IF staining. Objective: 40x.| Application Notes | WB:1:6000-1:24000 IHC:1:100-1:400 IF-F:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
