WB
Western Blot,Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20µg),Rat Skeletal Muscle tissue lysate(20µg),All lanes: TPST1 antibody at 1:1000,Secondary,Goat polyclonal to rabbit IgG at 1/40000 dilution,Exposure time: 120s.WB
Western Blot,Positive WB detected in: A549 whole cell lysate(20µg), U251 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Hela whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293 whole cell lysate(20µg),All lanes: TPST1 antibody at 1:1000,Secondary,Goat polyclonal to rabbit IgG at 1/20000 dilution,Exposure time: 60s.IHC
IHC image of TPST1 Antibody diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.| Product Name | TPST1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Protein-tyrosine sulfotransferase 1 protein (283-370AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50-1:200 WB:1:1000-1:3000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPST1 |
|---|---|
| Gene Synonyms | TANGO13A |
| Gene Full Name | tyrosylprotein sulfotransferase 1 |
| Gene Summary | Enables protein homodimerization activity and protein-tyrosine sulfotransferase activity. Involved in post-translational protein modification. Located in Golgi membrane. Implicated in colorectal adenocarcinoma. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TPST1 Protein-tyrosine sulfotransferase 1 Tyrosylprotein sulfotransferase 1 TPST-1 |
| Molecular Weight(MW) | 43kDa(Observed MW 43kDa) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. |
WB
Western Blot,Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20µg),Rat Skeletal Muscle tissue lysate(20µg),All lanes: TPST1 antibody at 1:1000,Secondary,Goat polyclonal to rabbit IgG at 1/40000 dilution,Exposure time: 120s.
WB
Western Blot,Positive WB detected in: A549 whole cell lysate(20µg), U251 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Hela whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293 whole cell lysate(20µg),All lanes: TPST1 antibody at 1:1000,Secondary,Goat polyclonal to rabbit IgG at 1/20000 dilution,Exposure time: 60s.
IHC
IHC image of TPST1 Antibody diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.| Application Notes | IHC:1:50-1:200 WB:1:1000-1:3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.02% sodium azide Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
