WB
Western Blot, Positive WB detected in: MCF7 whole cell lysate(30µg), COLO205 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U251 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg), All lanes: ZDHHC12 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.IHC
IHC image of ZDHHC12 Antibody diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.| Product Name | ZDHHC12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Probable palmitoyltransferase ZDHHC12 protein(65-140aa) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:20-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZDHHC12 |
|---|---|
| Gene Synonyms | DHHC12 ZNF400 DHHC-12 |
| Gene Full Name | zDHHC palmitoyltransferase 12 |
| Gene Summary | Enables protein-cysteine S-palmitoyltransferase activity. Involved in gephyrin clustering involved in postsynaptic density assembly; negative regulation of NLRP3 inflammasome complex assembly; and protein palmitoylation. Located in Golgi apparatus and endoplasmic reticulum. Is active in dendritic spine. [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 31/25/37 kDa(Observed MW 37kDa) |
| Cellular Localization | Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. |
WB
Western Blot, Positive WB detected in: MCF7 whole cell lysate(30µg), COLO205 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U251 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg), All lanes: ZDHHC12 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.
IHC
IHC image of ZDHHC12 Antibody diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.| Application Notes | WB:1:500-1:2000 IHC:1:20-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
