WB
Western Blot, Positive WB detected in: Hela whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), A549 whole cell lysate(20µg), 293 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)Mouse Brain tissue lysate(20µg), All lanes: GFRAL antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/20000 dilution, Exposure time: 30s.ICC/IF
Immunofluorescence staining of Hela cell with GFRAL Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | GFRAL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human GDNF family receptor alpha-like protein (19-351AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:2000 ICC/IF:1:20-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZDHHC12 |
|---|---|
| Gene Synonyms | GRAL UNQ9356 C6orf144 bA360D14.1 |
| Gene Full Name | GDNF family receptor alpha like |
| Gene Summary | Enables glial cell-derived neurotrophic factor receptor activity and receptor tyrosine kinase binding activity. Involved in GDF15-GFRAL signaling pathway; positive regulation of MAPK cascade; and positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Located in several cellular components, including actin cytoskeleton; focal adhesion; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 45 kDa(Observed MW 45kDa) |
| Cellular Localization | Cell membrane; Single-pass membrane protein; Extracellular side. |
WB
Western Blot, Positive WB detected in: Hela whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), A549 whole cell lysate(20µg), 293 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)Mouse Brain tissue lysate(20µg), All lanes: GFRAL antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/20000 dilution, Exposure time: 30s.
ICC/IF
Immunofluorescence staining of Hela cell with GFRAL Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:2000 ICC/IF:1:20-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
