WB
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Mouse skeletal muscle tissue All lanes: USP18 antibody at 2μg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human testis tissue using USP18 Antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of MCF-7 cells using USP18 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | USP18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Ubl carboxyl-terminal hydrolase 18 protein (18-118AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | USP18 |
|---|---|
| Gene Synonyms | ISG43 UBP43 PTORCH2 |
| Gene Full Name | ubiquitin specific peptidase 18 |
| Gene Summary | The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 44/42kDa(Observed MW 44kDa) |
| Cellular Localization | Nucleus. Cytoplasm. |
WB
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Mouse skeletal muscle tissue All lanes: USP18 antibody at 2μg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human testis tissue using USP18 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of MCF-7 cells using USP18 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
