WB
Western Blot, Positive WB detected in: RAW264.7 whole cell lysate(30µg), 293 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg), All lanes: FUNDC1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.WB
Western Blot, Positive WB detected in: Mouse Brain tissue lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Brain tissue lysate(30µg),Rat Liver tissue lysate(30µg), All lanes: FUNDC1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.IHC
IHC image of FUNDC1 Antibody diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.| Product Name | FUNDC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human FUN14 domain-containing protein 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FUNDC1 |
|---|---|
| Gene Full Name | FUN14 domain containing 1 |
| Gene Summary | This gene encodes a protein with a FUN14 superfamily domain. The function of the encoded protein is not known. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 17kDa(Observed MW 17kDa) |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein. |
WB
Western Blot, Positive WB detected in: RAW264.7 whole cell lysate(30µg), 293 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg), All lanes: FUNDC1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.
WB
Western Blot, Positive WB detected in: Mouse Brain tissue lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Brain tissue lysate(30µg),Rat Liver tissue lysate(30µg), All lanes: FUNDC1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Exposure time: 60s.
IHC
IHC image of FUNDC1 Antibody diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
