DB
Dot blot analysis was performed using Phospho-p-RIPK2(Ser176) Antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-RIPK2 (Ser176) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Ser176 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | RIPK2 |
|---|---|
| Gene Synonyms | CCK RICK RIP2 CARD3 GIG30 CARDIAK |
| Gene Full Name | receptor interacting serine/threonine kinase 2 |
| Gene Summary | This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008] |
| Alternative Names | CARD 3 CARD carrying kinase CARD containing ICE associated kinase CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD-containing IL-1 beta ICE-kinase CARD-containing interleukin-1 beta-converting enzyme-associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 Receptor-interacting protein 2 Receptor-interacting serine/threonine-protein kinase 2 RICK RIP 2 RIP like interacting CLARP kinase RIP-2 RIP-like-interacting CLARP kinase RIPK 2 Ripk2 RIPK2_HUMAN TNFRSF Tyrosine-protein kinase RIPK2 UNQ277/PRO314/PRO34092 |
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O43353 |
|---|
DB
Dot blot analysis was performed using Phospho-p-RIPK2(Ser176) Antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
