ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-RIPK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human RIPK2 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK2 |
|---|---|
| Gene Synonyms | CARD3 CARDIAK CCK GIG30 RICK RIP2 |
| Gene Full Name | receptor interacting serine/threonine kinase 2 |
| Gene Summary | This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008] |
| Alternative Names | CARD domain protein phosphorylated protein CARD-carrying kinase CARD-containing IL-1 beta ICE-kinase CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase CARDIAK RIP-like kinase EC 2.7.11.1 growth-in growth-inhibiting gene 30 kinase RIPK2 receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase receptor-interacting protein 2 RIP 2 RIP-2 RIP-like RIP2 serine/threonine kinase homolog contains CARD motif tyrosine-protein kinase RIPK2 WUGSC:H_RG437L15.1 |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O43353 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
