-DB-Figure-20260811_1.jpg)
DB
Dot blot analysis was performed using Anti-Phospho-CaMKII(Tyr231) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-CaMKII(Tyr231) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Tyr231 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CaMKII |
|---|---|
| Gene Synonyms | Ca2+/calmodulin-dependent protein kinase II Calcium/calmodulin-dependent protein kinase calcium/calmodulin-dependent protein kinase II Cam CaM CaM II CaM KII CaM kinase camk II CaMK II CamK-II CaMK-II camkii camkII camKII Camkii CamKII CaMKii CAMKII CamKIIalpha CAMKIIalpha CAMKIId CDPK1 CG18069 dCaMKII dCAMKII DCK Dmel\CG18069 l(4)102EFb l(4)16 l(4)ar |
| Gene Full Name | Calcium/calmodulin-dependent protein kinase II |
| Gene Summary | Enables calcium/calmodulin-dependent protein kinase activity. Involved in several processes, including long-term memory; negative regulation of lipid storage; and regulation of filopodium assembly. Acts upstream of with a positive effect on positive regulation of dopaminergic synaptic transmission. Located in several cellular components, including dendrite; postsynaptic membrane; and presynaptic active zone. Is expressed in several structures, including A1-7 ventral longitudinal muscle cell; adult brain; ganglia; midgut primordium; and synaptic neuropil domain. Used to study heart conduction disease. Human ortholog(s) of this gene implicated in autosomal dominant intellectual developmental disorder 53; autosomal dominant intellectual developmental disorder 54; autosomal dominant intellectual developmental disorder 59; and autosomal recessive intellectual developmental disorder 63. Orthologous to several human genes including CAMK2D (calcium/calmodulin dependent protein kinase II delta). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Calcium/calmodulin-dependent protein kinase type II subunit alpha Calcium/calmodulin-dependent protein kinase type II subunit delta CaM kinase II subunit alpha CaM kinase II subunit delta CAM2 CaMK-II subunit alpha CaMK-II subunit delta CAMK2A CAMK2B CAMKA CAMKB CAMKD CaMKII alpha KIAA0968 |
| Molecular Weight(MW) | 73kDa |
| Cellular Localization | Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse. |
| SwissProt ID | Q13554 |
|---|
DB
Dot blot analysis was performed using Anti-Phospho-CaMKII(Tyr231) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
