DB
Dot blot analysis was performed using Anti-Phospho-IKKgamma(Ser376) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-IKK gamma(Ser376) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Ser376 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKG |
|---|---|
| Gene Synonyms | IP IP1 IP2 FIP3 IKKG IPD2 NEMO FIP-3 Fip3p IMD33 SAIDX AMCBX1 EDAID1 IKKAP1 ZC2HC9 IKK-gamma |
| Gene Full Name | inhibitor of nuclear factor kappa B kinase regulatory subunit gamma |
| Gene Summary | This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016] |
| Alternative Names | IkB kinase associated protein 1 IkB kinase subunit gamma Inhibitor of nuclear factor kappa B kinase subunit gamma AMCBX1 FIP 3 FIP-3 FIP3 Fip3p I kappa B kinase gamma I-kappa-B kinase subunit gamma IkB kinase gamma subunit IkB kinase subunit gamma IkB kinase-associated protein 1 Ikbkg IKK-gamma IKKAP1 IKKG IMD33 Incontinentia pigmenti Inhibitor of kappa light polypeptide gene enhancer in B cells kinase gamma Inhibitor of kappa light polypeptide gene enhancer in B cells kinase of gamma Inhibitor of nuclear factor kappa-B kinase subunit gamma IP IP1 IP2 IPD2 NEMO NEMO_HUMAN NF kappa B essential modifier NF kappa B essential modulator NF-kappa-B essential modifier NF-kappa-B essential modulator ZC2HC9 |
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm,Nucleus |
| SwissProt ID | Q9Y6K9 |
|---|
DB
Dot blot analysis was performed using Anti-Phospho-IKKgamma(Ser376) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
