DB
Dot blot analysis was performed using Anti-Phospho-DLGAP1(Ser346) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-DLGAP1(Ser346) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Ser346 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLGAP1 |
|---|---|
| Gene Synonyms | DAP1 GKAP DAP-1 SAPAP1 DLGAP1A DLGAP1B DAP-1-BETA DAP-1-ALPHA |
| Gene Full Name | DLG associated protein 1 |
| Gene Summary | Predicted to enable molecular adaptor activity. Predicted to be a structural constituent of postsynaptic density. Predicted to be involved in several processes, including aggresome assembly; protein localization to synapse; and regulation of proteasomal protein catabolic process. Predicted to be located in plasma membrane. Predicted to be active in glutamatergic synapse and postsynaptic density, intracellular component. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 4933422O14Rik 9630002F18 AI845682 AI848168 BB075781 D17Bwg0511e DAP 1 ALPHA DAP 1 DAP 1 BETA DAP-1 DAP-1-ALPHA DAP-1-BETA DAP1 Discs large (Drosophila) homolog associated protein 1 Discs large homolog associated protein 1 Disks large associated protein 1 Disks large associated protein 1 alpha isoform included Disks large associated protein 1 beta isoform included Disks large-associated protein 1 DLGAP 1 Dlgap1 DLGP1_HUMAN GKAP GKAP/SAPAP GKPA/SAPAP Guanylate kinase associated protein Guanylate kinase-associated protein hGKAP KIAA4162 MGC88156 mKIAA4162 PSD 95/SAP90 binding protein 1 PSD-95/SAP90-binding protein 1 SAP90/PSD 95 associated protein 1 SAP90/PSD-95-associated protein 1 SAPAP 1 SAPAP1 |
| Molecular Weight(MW) | 109 kDa |
| Cellular Localization | Cell membrane>Peripheral membrane protein. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. |
| SwissProt ID | O14490 |
|---|
DB
Dot blot analysis was performed using Anti-Phospho-DLGAP1(Ser346) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
