WB
Western blot with Anti-Phospho-EIF3B(Ser154) antibody antibody on lysates of mouse muscle tissue (1) .12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Product Name | Anti-Phospho-EIF3B(Ser154) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Ser154 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF3B |
|---|---|
| Gene Synonyms | PRT1 EIF3S9 EIF3-ETA EIF3-P110 EIF3-P116 eIF3B |
| Gene Full Name | eukaryotic translation initiation factor 3 subunit B |
| Gene Summary | Translation initiation requires a set of factors to facilitate the association of the 40S ribosomal subunit with mRNA. The eIF4F complex, consisting of eIF4E, eIF4A, and eIF4G, binds to the 5' cap structure of mRNA. eIF4F and eIF4B unwind the secondary structure of mRNA at its 5' untranslated region |
| Alternative Names | eIF-3-eta eIF3 eta eIF3 p110 eIF3 p116 EIF3-ETA EIF3-P110 EIF3-P116 eIF3b EIF3B_HUMAN EIF3S9 EIF3S9 formerly Eukaryotic translation initiation factor 3 subunit B Eukaryotic translation initiation factor 3 subunit 9 eukaryotic translation initiation factor 3 subunit 9 eta Eukaryotic translation initiation factor 3 subunit B eukaryotic translation initiation factor 3 subunit 9 (eta 116kD) eukaryotic translation initiation factor 3 subunit 9 eta 116kDa eukaryotic translation initiation factor 3 subunit 9 formerly hPrt1 Protein synthesis defective at 36 degrees celsius 1 S. cerevisiae homolog of PRT1 Prt1 homolog |
| Molecular Weight(MW) | 92kDa(Predicted); 105kDa(Observed） |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P55884 |
|---|
WB
Western blot with Anti-Phospho-EIF3B(Ser154) antibody antibody on lysates of mouse muscle tissue (1) .12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
