WB
Western blot analysis using Anti-IL36RA-Human antibody against Recombinant IL36RA-Human protein. 15% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-IL36RA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human IL36RN protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL36RN |
|---|---|
| Gene Synonyms | FIL1 FIL1(DELTA) FIL1D Fil1delta If36rn Il-1h3 IL-36Ra IL1F5 IL1HY1 IL1L1 IL1RP3 IL36RA PSORP PSORS14 |
| Gene Full Name | interleukin 36 receptor antagonist |
| Gene Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | cytokine mIL-1H3 IL-1 delta IL-1 related protein 3 IL-1delta IL-1F5 IL-1F5 (IL-1HY1 FIL1-delta IL-1RP3 IL-1L1 IL-1-delta) IL-1HY1 IL-1L1 IL-1ra homolog IL-1ra homolog 1 IL1F5 (Canonical product IL-1F5a) Interleukin interleukin 1 family member 5 (delta) interleukin 1 receptor antagonist homolog 1 interleukin-1 delta interleukin-1 family member 5 interleukin-1 homolog 3 interleukin-1 HY1 interleukin-1 receptor antagonist homolog 1 interleukin-1-like protein 1 MGC29840 similar to IL-1 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cytoplasm, Secreted. |
| SwissProt ID | Q9UBH0 |
|---|
WB
Western blot analysis using Anti-IL36RA-Human antibody against Recombinant IL36RA-Human protein. 15% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
