ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-Phospho-Aurora A (Thr288) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Thr288 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AURKA |
|---|---|
| Gene Synonyms | AIK ARK1 AURA BTAK STK6 STK7 STK15 PPP1R47 |
| Gene Full Name | aurora kinase A |
| Gene Summary | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | AIK ARK-1 ARK1 AURA Aurka Aurora 2 Aurora A Aurora kinase A Aurora-related kinase 1 Aurora/IPL1 like kinase Aurora/IPL1-related kinase 1 AURORA2 Breast tumor-amplified kinase BTAK hARK1 IAK IPL1 related kinase MGC34538 OTTHUMP00000031340 OTTHUMP00000031341 OTTHUMP00000031342 OTTHUMP00000031343 OTTHUMP00000031344 OTTHUMP00000031345 OTTHUMP00000166071 OTTHUMP00000166072 PPP1R47 Protein phosphatase 1 regulatory subunit 47 Serine/threonine kinase 15 Serine/threonine kinase 6 Serine/threonine-protein kinase 15 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A STK15 STK6 STK6_HUMAN STK7 |
| Molecular Weight(MW) | 46kDa |
| Cellular Localization | Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. |
| SwissProt ID | O14965 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
