ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideWB
Western blot with Anti-Phospho-GATA1(Ser142) antibody on lysates of mouse liver (1).12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Product Name | Anti-Phospho-GATA1(Ser142) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser142 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA1 |
|---|---|
| Gene Synonyms | GF1 GF-1 NFE1 XLTT ERYF1 NF-E1 XLANP XLTDA CNSHA9 GATA-1 HAEADA |
| Gene Full Name | GATA binding protein 1 |
| Gene Summary | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008] |
| Alternative Names | Anemia X-linked without thrombocytopenia included antibody ERYF 1 antibody Eryf1 antibody Erythroid transcription factor antibody Erythrold transcription factor 1 antibody GATA 1 antibody GATA binding factor 1 antibody GATA binding protein 1 (globin transcription factor 1) antibody GATA binding protein 1 antibody GATA-1 antibody GATA-binding factor 1 antibody GATA1 antibody GATA1_HUMAN antibody GF 1 antibody GF-1 antibody GF1 antibody Globin transcription factor 1 antibody NF E1 antibody NF E1 DNA binding protein antibody NF-E1 DNA-binding protein antibody NFE 1 antibody NFE1 antibody Nuclear factor erythroid 1 antibody Transcription factor GATA1 antibody XLANP antibody XLTDA antibody XLTT antibody |
| Molecular Weight(MW) | 43kDa(Predicted); 43kDa(Observed） |
| Cellular Localization | Nucleus. |
| SwissProt ID | P15976 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
WB
Western blot with Anti-Phospho-GATA1(Ser142) antibody on lysates of mouse liver (1).12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
