ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-p44/42 MAPK(Erk1/2) (Thr202) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr202 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK1/MAPK3 |
|---|---|
| Gene Synonyms | ERK p38 p40 p41 ERK2 ERT1 NS13 ERK-2 MAPK2 PRKM1 PRKM2 P42MAPK p41mapk p42-MAPK ERK1 ERT2 ERK-1 PRKM3 P44ERK1 P44MAPK HS44KDAP HUMKER1A p44-ERK1 p44-MAPK |
| Gene Full Name | mitogen-activated protein kinase 1/mitogen-activated protein kinase 3 |
| Gene Summary | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |
| Alternative Names | ERK 1 ERK ERK-1 ERK1 ERT 2 ERT2 Extracellular Signal Regulated Kinase 1 Extracellular signal related kinase 1 Extracellular signal-regulated kinase 1 HGNC6877 HS44KDAP HUMKER1A Insulin Stimulated MAP2 Kinase Insulin-stimulated MAP2 kinase MAP kinase 1 MAP kinase 3 MAP Kinase MAP kinase isoform p44 MAPK 1 MAPK 3 MAPK MAPK1 Mapk3 MGC20180 Microtubule Associated Protein 2 Kinase Microtubule-associated protein 2 kinase Mitogen Activated Protein Kinase 3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 3 MK03_HUMAN OTTHUMP00000174538 OTTHUMP00000174541 p44 ERK1 p44 MAPK p44-ERK1 p44-MAPK P44ERK1 P44MAPK PRKM 3 PRKM3 Protein Kinase Mitogen Activated 3 ERK 2 ERK ERK-2 ERT1 Extracellular Signal Regulated Kinase 2 Extracellular signal-regulated kinase 2 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAPK 1 MAPK 2 Mapk1 MAPK2 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 MK01_HUMAN P38 P40 P41 p42-MAPK P42MAPK PRKM1 PRKM2 protein kinase mitogen-activated 1 protein kinase mitogen-activated 2 protein tyrosine kinase ERK2 |
| Molecular Weight(MW) | 43kDa,41kDa |
| Cellular Localization | Cell junction, Cytoplasm, Cytoskeleton, Membrane, Nucleus |
| SwissProt ID | P27361/P28482 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
