ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-MST1 (Thr183)/MST2 (Thr180) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-MST1 (Thr183)/MST2 (Thr180) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr183/Thr180 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK4/STK3 |
|---|---|
| Gene Synonyms | KRS2 MST1 YSK3/KRS1 MST2 |
| Gene Full Name | serine/threonine kinase 4/serine/threonine kinase 3 |
| Gene Summary | The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008] |
| Alternative Names | Kinase responsive to stress Krs2 Mammalian STE20 like protein kinase 1 Mammalian STE20-like protein kinase 1 Mammalian sterile 20 like 1 MST-1 MST1 Serine/threonine kinase 4 Serine/threonine protein kinase Krs 2 Serine/threonine-protein kinase 4 Serine/threonine-protein kinase Krs-2 STE20 like kinase MST1 STE20-like kinase MST1 STK4 STK4_HUMAN TIIAC YSK3 0610042I06Rik EC 2.7.11.1 FLJ90748 KB 1458E12.1 Kinase responsive to stress 1 KRS1 Mammalian STE20 like protein kinase 2 Mammalian STE20-like protein kinase 2 Mammalian sterile 20-like 2 Mess1 MST MST-2 MST2 Mst3 Serine/threonine kinase 3 (STE20 homolog yeast) Serine/threonine kinase 3 (Ste20 yeast homolog) Serine/threonine kinase 3 Serine/threonine protein kinase 3 Serine/threonine protein kinase Krs1 Serine/threonine-protein kinase 3 Serine/threonine-protein kinase Krs-1 STE20 like kinase MST2 STE20-like kinase MST2 Stk3 STK3_HUMAN wu:fc19e11 zgc:55383 |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm,Nucleus./Cytoplasm,Cytoskeleton,Nucleus. |
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-MST1 (Thr183)/MST2 (Thr180) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
