ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-EPHA2/3/4(Tyr588/Tyr596) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-EPHA2/3/4(Tyr588/Tyr596) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr588/Tyr596 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPHA2/EPHA3/EPHA4 |
|---|---|
| Gene Synonyms | ECK CTPA ARCC2 CTPP1 CTRCT6/EK4 ETK HEK ETK1 HEK4 TYRO4/EK8 SEK HEK8 TYRO1 |
| Gene Full Name | EPH receptor A2/EPH receptor A3/EPH receptor A4 |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010] |
| Alternative Names | ARCC2 AW545284 CTPA CTPP1 CTRCT6 EC 2.7.10.1 Eck Eph receptor A2 EPHA2 EPHA2_HUMAN Ephrin receptor Ephrin receptor EphA2 Ephrin type A receptor 2 Ephrin type-A receptor 2 Epithelial cell kinase Epithelial cell receptor protein tyrosine kinase Myk 2 Myk2 Sek 2 Sek2 Soluble EPHA2 variant 1 Tyrosine protein kinase receptor ECK Tyrosine-protein kinase receptor ECK Tyrosine-protein kinase receptor MPK-5 Tyrosine-protein kinase receptor SEK-2 AW492086 Cek4 EC 2.7.10.1 EK4 End3 Eph receptor A3 EPH-like kinase 4 EPH-like tyrosine kinase 1 EPHA3 EPHA3_HUMAN Ephrin receptor EphA3 Ephrin type-A receptor 3 ETK 1 ETK ETK1 HEK 4 HEK HEK4 Human embryo kinase 1 Human embryo kinase Mek4 MGC109882 Receptor tyrosine kinase HEK Testicular tissue protein Li 64 Tyro 4 Tyro4 TYRO4 protein tyrosine kinase Tyrosine protein kinase receptor ETK 1 Tyrosine-protein kinase receptor ETK1 Tyrosine-protein kinase TYRO4 Cek 8 CEK8 EK8 eph receptor a4 EPH-like kinase 8 EPHA4 EPHA4_HUMAN Ephrin type-A receptor 4 HEK 8 hEK8 Receptor protein-tyrosine kinase HEK8 Sek 1 SEK TYRO 1 protein tyrosine kinase TYRO1 Tyrosine-protein kinase receptor SEK Tyrosine-protein kinase TYRO1 |
| Molecular Weight(MW) | 108 kDa,110 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Membrane./ Cell membrane,Membrane,Secreted./ Cell junction,Cell membrane,Cell projection,Endosome,Membrane,Postsynaptic cell membrane,Synapse. |
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-EPHA2/3/4(Tyr588/Tyr596) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
