DB
Dot blot analysis was performed using Anti-Phospho-BAP1(Ser592) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-BAP1(Ser592) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser592 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAP1 |
|---|---|
| Gene Synonyms | UBM2 UVM2 KURIS TPDS1 UCHL2 hucep-6 HUCEP-13 |
| Gene Full Name | BRCA1 associated deubiquitinase 1 |
| Gene Summary | This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013] |
| Alternative Names | BAP 1 Bap1 BAP1_HUMAN BRCA 1 associated protein 1 BRCA1 associated protein 1 BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) BRCA1-associated protein 1 Cerebral protein 13 Cerebral protein 6 DKFZp686N04275 FLJ35406 FLJ37180 HUCEP 13 Hucep 6 HUCEP13 Hucep6 KIAA0272 TPDS Ubiquitin carboxy terminal hydrolase Ubiquitin carboxyl terminal hydrolase BAP 1 Ubiquitin carboxyl terminal hydrolase BAP1 Ubiquitin carboxyl-terminal hydrolase BAP1 UCHL2 |
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus. |
| SwissProt ID | Q92560 |
|---|
DB
Dot blot analysis was performed using Anti-Phospho-BAP1(Ser592) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
