ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-Histone H3(Ser11) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-Histone H3 (Ser11) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C13/H3C14/H3C15 |
|---|---|
| Gene Synonyms | H3 H3.2 H3/M H3F2 H3FM H3FN HIST2H3C H3/n H3/o HIST2H3A HIST2H3D |
| Gene Full Name | H3 clustered histone 13/H3 clustered histone 14/H3 clustered histone 15 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3 histone family member A H3/A H31_HUMAN H3FA Hist1h3a HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J histone 1 H3a Histone cluster 1 H3a Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3.3A HIST1 cluster H3E H3 histone family member A H3.1 H3/l H3F3 H3FF H3FJ H3FL Histone gene cluster 1 H3 histone family member E histone H3.1t Histone H3/o FLJ92264 H 3 H3 H3 histone family member B H3 histone family member C H3 histone family member D H3 histone family member F H3 histone family member H H3 histone family member I H3 histone family member J H3 histone family member K H3 histone family member L H3 histone family member T H3 histone family 3A H3/A H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/t H31_HUMAN H3F1K H3F3A H3FA H3FB H3FC H3FD H3FH H3FI H3FK HIST1 cluster H3A HIST1 cluster H3B HIST1 cluster H3C HIST1 cluster H3D HIST1 cluster H3F HIST1 cluster H3G HIST1 cluster H3H HIST1 cluster H3I HIST1 cluster H3J HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST3H3 Histone 1 H3a Histone 1 H3b Histone 1 H3c Histone 1 H3d Histone 1 H3e Histone 1 H3f Histone 1 H3g Histone 1 H3h Histone 1 H3i Histone 3 H3 histone cluster 1 H3 family member a histone cluster 1 H3 family member b histone cluster 1 H3 family member c histone cluster 1 H3 family member d histone cluster 1 H3 family member e histone cluster 1 H3 family member f histone cluster 1 H3 family member g histone cluster 1 H3 family member h histone cluster 1 H3 family member i histone cluster 1 H3 family member j Histone cluster 1 H3a Histone cluster 1 H3b Histone cluster 1 H3c Histone cluster 1 H3d Histone cluster 1 H3e Histone cluster 1 H3f Histone cluster 1 H3g Histone cluster 1 H3i Histone cluster 1 H3j Histone gene cluster 1 H3 histone family member A Histone gene cluster 1 H3 histone family member B Histone gene cluster 1 H3 histone family member C Histone gene cluster 1 H3 histone family member D Histone gene cluster 1 H3 histone family member F Histone gene cluster 1 H3 histone family member G Histone gene cluster 1 H3 histone family member H Histone gene cluster 1 H3 histone family member I Histone gene cluster 1 H3 histone family member J Histone gene cluster 1 H3A Histone gene cluster 1 H3B Histone gene cluster 1 H3C Histone gene cluster 1 H3D Histone gene cluster 1 H3E Histone gene cluster 1 H3F Histone gene cluster 1 H3G Histone gene cluster 1 H3H Histone gene cluster 1 H3I Histone gene cluster 1 H3J Histone H 3 Histone H3.1 Histone H3.2 Histone H3.3 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l Histone H3/m H3 histone family 3A H3 histone family 3B H3 histone family 3B (H3.3B) H3.3 H3.3A H3.3B H33_HUMAN H3F3 H3F3A H3f3b Histone H3.3 Histone H3.3Q Histone H3.A Histone H3.B MGC87782 MGC87783 |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus. |
| SwissProt ID | Q71DI3 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-Histone H3(Ser11) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
