ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-RUNX1/AML1(Ser276) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-RUNX1 / AML1 (Ser276) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser276 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUNX1 |
|---|---|
| Gene Synonyms | AML1 CBFA2 EVI-1 AMLCR1 PEBP2aB CBF2alpha AML1-EVI-1 PEBP2alpha |
| Gene Full Name | RUNX family transcription factor 1 |
| Gene Summary | Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Acute myeloid leukemia 1 Acute myeloid leukemia 1 protein alpha subunit core binding factor AML 1 AML1 AML1 EVI 1 AML1 EVI 1 fusion protein Aml1 oncogene AMLCR 1 AMLCR1 CBF alpha 2 CBF-alpha-2 CBFA 2 CBFA2 Core binding factor alpha 2 subunit Core binding factor runt domain alpha subunit 2 Core-binding factor subunit alpha-2 EVI 1 EVI1 HGNC Oncogene AML 1 Oncogene AML-1 OTTHUMP00000108696 OTTHUMP00000108697 OTTHUMP00000108699 OTTHUMP00000108700 OTTHUMP00000108702 PEA2 alpha B PEA2-alpha B PEBP2 alpha B PEBP2-alpha B PEBP2A2 PEBP2aB Polyomavirus enhancer binding protein 2 alpha B subunit Polyomavirus enhancer-binding protein 2 alpha B subunit Run1 Runt related transcription factor 1 Runt-related transcription factor 1 RUNX 1 Runx1 RUNX1_HUMAN SL3 3 enhancer factor 1 alpha B subunit SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core binding factor alpha B subunit SL3/AKV core-binding factor alpha B subunit |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q01196 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-RUNX1/AML1(Ser276) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
