ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-DARPP32 (Thr75) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1R1B |
|---|---|
| Gene Synonyms | DARPP32 DARPP-32 |
| Gene Full Name | protein phosphatase 1 regulatory inhibitor subunit 1B |
| Gene Summary | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | DARPP 32 DARPP-32 Dopamine and cAMP regulated neuronal phosphoprotein 32 Dopamine and cAMP regulated neuronal phosphoprotein Dopamine and cAMP regulated phosphoprotein Dopamine and cAMP regulated phosphoprotein DARPP 32 Dopamine and cAMP regulated phosphoprotein DARPP32 Dopamine- and cAMP-regulated neuronal phosphoprotein FLJ20940 IPPD Neuronal phosphoprotein DARPP 32 PPP1R1B PPR1B_HUMAN Protein phosphatase 1 regulatory (inhibitor) subunit 1B Protein phosphatase 1 regulatory inhibitor subunit 1B Protein phosphatase 1 regulatory subunit 1B |
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9UD71 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
