WB
Western blot analysis of NF-M in Hela , NIH/3T3 lysates and using NF-M Goat pAb.IF
Immunofluorescence analysis of NF-M(Green) in mouse brain tissue using NF-M Goat pAb, and DAPI(Blue)| Product Name | 160 kD Neurofilament Medium Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human NF-M |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | NEFM |
|---|---|
| Gene Synonyms | NFM NEF3 NF-M |
| Gene Full Name | neurofilament medium chain |
| Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Molecular Weight(MW) | 140 kDa(Observed band size: 130 kDa) |
| Function | Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Intermediate filament |
WB
Western blot analysis of NF-M in Hela , NIH/3T3 lysates and using NF-M Goat pAb.
IF
Immunofluorescence analysis of NF-M(Green) in mouse brain tissue using NF-M Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
