WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-M (Phospho Ser614/Ser619) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-M (Phospho Ser614/Ser619) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human brain was stained with anti-NF-M (Phospho Ser614/Ser619) Rabbit antibody.| Product Name | NF-M(Phospho Ser614/Ser619) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Ser614/Ser619 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEFM |
|---|---|
| Gene Synonyms | NFM NEF3 NF-M |
| Gene Full Name | neurofilament medium chain |
| Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Alternative Names | NEFM NEF3 NFM Neurofilament medium polypeptide NF-M 160 kDa neurofilament protein Neurofilament 3 Neurofilament triplet M protein |
| Molecular Weight(MW) | 103 kDa(Observed band size: 180 kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton . Cell projection, axon . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-M (Phospho Ser614/Ser619) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-M (Phospho Ser614/Ser619) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human brain was stained with anti-NF-M (Phospho Ser614/Ser619) Rabbit antibody.| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
