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WB
Western blot analysis of S100B in mouse brain , rat brain lysates and using S100B Goat pAb.IF
Immunofluorescence analysis of S100B(Green) in mouse brain tissue using S100B Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human breast tissue sections using the antibody S100B Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | S100 beta Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human S100B |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | S100B |
|---|---|
| Gene Synonyms | NEF S100 S100-B S100beta |
| Gene Full Name | S100 calcium binding protein B |
| Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 11 kDa |
| Function | S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. |
| Cellular Localization | Cytoplasm,Nucleus,Secreted |
WB
Western blot analysis of S100B in mouse brain , rat brain lysates and using S100B Goat pAb.
IF
Immunofluorescence analysis of S100B(Green) in mouse brain tissue using S100B Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human breast tissue sections using the antibody S100B Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
