WB
Western blot analysis of anti-S100 Beta/S100B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100 Beta/S100B antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody.IHC
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody.IHC
Immunohistochemical analysis of paraffin-embedded Mouse pancreas, using the Antibody.ICC/IF
Immunofluorescent analysis using the Antibody.| Product Name | Rabbit monoclonal antibody to S100 Beta/S100B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human S100B. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-200 ICC/IF:1:50-200 |
|
| Species Reactivity | GoatHumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S100B |
|---|---|
| Gene Synonyms | NEF S100 S100-B S100beta |
| Gene Full Name | S100 calcium binding protein B |
| Gene Summary | he protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008] |
| Alternative Names | NEF Protein S100 B S 100 protein beta chain S 100 protein subunit beta S100 S100 Beta S100 calcium binding protein B S100B S100beta |
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Cytoplasm,Nucleus,Secreted. |
WB
Western blot analysis of anti-S100 Beta/S100B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100 Beta/S100B antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody.
IHC
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse pancreas, using the Antibody.
ICC/IF
Immunofluorescent analysis using the Antibody.| Application Notes | WB:1:500-2000 IHC:1:50-200 ICC/IF:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol. |
