Application
Below: Immunohistochemical analysis of paraffin - embedded human brain tissue (A) and human thymus tissue (B), showing cytoplasmic localization using anti - S100B antibody with DAB staining.Application
Western blot analysis using anti - S100B monoclonal antiobdy against full - length S100B recombinant protein.| Product Name | S100B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S-100 protein beta chainNEF, S100, S100-B, S100beta |
| Product description | S100B (S100 calcium binding protein B) is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S100B are present in a variety of other tissues, and calbindin is present in intestine and kidney. Parvalbumin B is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.1) Shapiro LA. Marks A. Whitaker-Azmitia PM. Brain Res. 2004, Jun 4,1010(1-2):17-21. |
| Immunogen | Purified full-length recombinant S100B-GST expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 bIHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | S100B |
|---|---|
| Alternative Names | S-100 protein beta chainNEF S100 S100-B S100beta |
| Sequence Similarities | 2) Cat. No. 1314 - Human Thymus Tissue Lysate |
Application
Below: Immunohistochemical analysis of paraffin - embedded human brain tissue (A) and human thymus tissue (B), showing cytoplasmic localization using anti - S100B antibody with DAB staining.
Application
Western blot analysis using anti - S100B monoclonal antiobdy against full - length S100B recombinant protein.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 bIHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | S100B monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
