WB
Western Blot,Samples: Lane1: Hela cell lysate,Lane2: HUVEC cell lysate,Lane3: MDA-MB-231 cell lysate;Primary Ab: 0.2µg/ml Mouse Anti-Human HCAM AntibodySecond Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyFC
Human peripheral blood lymphocytes were stained with 10µg/ml mouse Anti-human HCAM Monoclonal Antibody (green histogram) or Isotype control antibody (blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody.| Product Name | Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | EPA670Hu62-Eukaryotic Homing Associated Cell Adhesion Molecule (HCAM) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1Kappa |
| Host Species | Mouse |
| Tested Applications | WBFCM |
| WB: 0.01-2µg/mL Flow cytometry: 10µg/mL |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | PDE1A |
|---|---|
| Gene Synonyms | HCAM1,HCAM-1,HSPDE1A,CAM-PDE 1A,CAM-PDE-1A |
| Gene Full Name | phosphodiesterase 1A |
| Gene Summary | Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1,MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109],Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] |
| Alternative Names | CDW44 CD44 CSPG8 ECMR-III HCELL IN LHR MC56 MDU2 MDU3 MIC4 MUTCH-I Pgp1 Hematopoietic Cell E- And L-Selectin Ligand Chondroitin Sulfate Proteoglycan 8 |
WB
Western Blot,Samples: Lane1: Hela cell lysate,Lane2: HUVEC cell lysate,Lane3: MDA-MB-231 cell lysate;Primary Ab: 0.2µg/ml Mouse Anti-Human HCAM AntibodySecond Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
FC
Human peripheral blood lymphocytes were stained with 10µg/ml mouse Anti-human HCAM Monoclonal Antibody (green histogram) or Isotype control antibody (blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody.| Application Notes | WB: 0.01-2µg/mL Flow cytometry: 10µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
