Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1,MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109],Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
Western Blot,Samples: Lane1: Hela cell lysate,Lane2: A549 cell lysate,Primary Ab: 0.2µg/ml Mouse Anti-Human HCAM Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Application Notes
WB: 0.01-2µg/mL
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.