ICC/IF
Immunofluorescence staining of Hela cells with Rabbit anti-PDE1A Polyclonal antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-PDE1A Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A protein (1-219AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PDE1A |
|---|---|
| Gene Synonyms | HCAM1 HCAM-1 HSPDE1A CAM-PDE 1A CAM-PDE-1A |
| Gene Full Name | phosphodiesterase 1A |
| Gene Summary | Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] |
| Alternative Names | 3' 5' cyclic nucleotide phosphodiesterase antibody 5''-cyclic nucleotide phosphodiesterase 1A antibody 61 kDa Cam PDE antibody 61 kDa Cam-PDE antibody Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A antibody Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase antibody Calcium/calmodulin-dependent 3'' antibody Calmodulin-dependent Phosphodiesterase antibody Cam PDE 1A antibody Cam-PDE 1A antibody CAM-PDE-1A antibody HCAM 1 antibody hCam-1 antibody HCAM1 antibody HSPDE1A antibody MGC26303 antibody PDE 1A antibody PDE1A antibody PDE1A_HUMAN antibody Phosphodiesterase 1A antibody Phosphodiesterase 1A calmodulin dependent antibody |
ICC/IF
Immunofluorescence staining of Hela cells with Rabbit anti-PDE1A Polyclonal antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
