Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
Alternative Names
T-PLASTIN
Application
ICC/IF
AF488 staining on IF;Sample: Hela cellPrimary Ab: 30µg/ml Mouse Anti-Human PLS3 AntibodySecond Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Application Notes
ICC/IF: 5-30µg/mL
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.