WB
Various lysates were subjected to SDS PAGE followed by western blot with PLS3 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using PLS3 antibody at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-PLS3 (4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2mg.| Product Name | PLS3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | PLS3 Fusion Protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:8000 IHC:1:400-1:1600 ICC:1:50-1:500 IP:0.5-4.0ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | PLS3 |
|---|---|
| Gene Synonyms | DIH5 BMND18 T-plastin |
| Gene Full Name | plastin 3 |
| Gene Summary | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010] |
| Alternative Names | T-plastin T-fimbrin T plastin Plastin-3 Plastin 3 |
| Molecular Weight(MW) | 71kDa(Observed Molecular Weight 68kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PLS3 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using PLS3 antibody at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-PLS3 (4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2mg.| Application Notes | WB:1:1000-1:8000 IHC:1:400-1:1600 ICC:1:50-1:500 IP:0.5-4.0ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Buffer | Store at -20°C. Stable for one year after shipment. |
