ICC/IF
Immunofluorescence analysis of C2C12 cells using CYP11A1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CYP11A1 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Ion Exchange Chromatography |
| Gene Symbol | CYP11A1 |
|---|---|
| Gene Synonyms | cscc CYP11A CYPXIA1 D9Ertd411e P450(scc) P450SCC Scc SSC |
| Gene Full Name | cytochrome P450 family 11 subfamily A member 1 |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] |
| Alternative Names | cholesterol 20-22 desmolase cholesterol desmolase cholesterol monooxygenase (side-chain cleaving) cholesterol side-chain cleavage cytochrome P450 cholesterol side-chain cleavage enzyme P450scc (EC 1.14.15.67) cholesterol side-chain cleavage enzyme precursor (EC 1.14.15.6) CYPXIA1 cytochrome P-450 cholesterol side-chain cleavage enzyme cytochrome P450 11a cholesterol side chain cleavage cytochrome P450 11A1 cytochrome P450 family 11 subfamily a cytochrome P450 family 11 subfamily A polypeptide 1 cytochrome P450 side-chain cleavage enzyme cytochrome P450 subfamily 11A cytochrome P450(scc) cytochrome P450 11a cholesterol side-chain cleavage cytochrome P450 subfamily XIA (cholesterol side chain cleavage) P450 cholesterol side chain cleavage protein P450scc steroidogenic enzyme cholesterol side chain cleavage enzyme steroid 20-22-lyase |
| Cellular Localization | Membrane, Mitochondrion, Mitochondrion inner membrane |
| SwissProt ID | P05108 |
|---|
ICC/IF
Immunofluorescence analysis of C2C12 cells using CYP11A1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
